A practical introduction to bioinformatics and RNA-seq using Galaxy

Learn how to interpret DESeq2 output, annotate and visualize differentially expressed genes, and conduct gene ontology and KEGG pathway enrichment analyses.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:00:55.800","2":"696dda4be4b0e8ba829faf99","3":"0.0","isRemoved":false},{"0":"00:00:55.800","1":"00:01:42.240","2":"696dda4be4b0e8ba829faf9b","3":"55.800000000000004","isRemoved":false},{"0":"00:01:42.240","1":"00:02:50.400","2":"696dda4be4b0e8ba829faf9d","3":"102.24000000000001","isRemoved":false},{"0":"00:02:50.400","1":"00:03:17.400","2":"696dda4be4b0e8ba829faf9f","3":"170.4","isRemoved":false},{"0":"00:03:17.400","1":"00:03:48.040","2":"696dda4be4b0e8ba829fafa1","3":"197.4","isRemoved":false},{"0":"00:03:48.040","1":"00:04:31.840","2":"696dda4be4b0e8ba829fafa3","3":"228.04000000000002","isRemoved":false},{"0":"00:04:31.840","1":"00:05:08.160","2":"696dda4be4b0e8ba829fafa5","3":"271.84000000000003","isRemoved":false},{"0":"00:05:08.160","1":"00:05:41.040","2":"696dda4be4b0e8ba829fafa7","3":"308.16","isRemoved":false},{"0":"00:05:41.040","1":"00:06:02.880","2":"696dda4be4b0e8ba829fafa9","3":"341.04","isRemoved":false},{"0":"00:06:02.880","1":"00:06:40.640","2":"696dda4be4b0e8ba829fafab","3":"362.88000000000005","isRemoved":false},{"0":"00:06:40.640","1":"00:08:13.240","2":"696dda4be4b0e8ba829fafad","3":"400.64000000000004","isRemoved":false},{"0":"00:08:13.240","1":"00:14:23.040","2":"696dda4be4b0e8ba829fafaf","3":"493.24000000000007","isRemoved":false},{"0":"00:14:23.040","1":"00:15:04.680","2":"696dda4be4b0e8ba829fafb1","3":"863.0400000000001","isRemoved":false},{"0":"00:15:04.680","1":"00:15:40.160","2":"696dda4be4b0e8ba829fafb3","3":"904.6800000000001","isRemoved":false},{"0":"00:15:40.160","1":"00:16:23.360","2":"696dda4be4b0e8ba829fafb5","3":"940.1600000000001","isRemoved":false},{"0":"00:16:23.360","1":"00:16:45.520","2":"696dda4be4b0e8ba829fafb7","3":"983.3600000000001","isRemoved":false},{"0":"00:16:45.520","1":"00:17:17.520","2":"696dda4be4b0e8ba829fafb9","3":"1005.5200000000001","isRemoved":false},{"0":"00:17:17.520","1":"00:19:26.240","2":"696dda4be4b0e8ba829fafbb","3":"1037.52","isRemoved":false},{"0":"00:19:26.240","1":"00:20:52.840","2":"696dda4be4b0e8ba829fafbd","3":"1166.24","isRemoved":false},{"0":"00:20:52.840","1":"00:21:26.280","2":"696dda4be4b0e8ba829fafbf","3":"1252.8400000000001","isRemoved":false},{"0":"00:21:26.280","1":"00:22:01.400","2":"696dda4be4b0e8ba829fafd5","3":"1286.2800000000002","isRemoved":false},{"0":"00:22:01.400","1":"00:22:21.600","2":"696dda4be4b0e8ba829fafd7","3":"1321.4","isRemoved":false},{"0":"00:22:21.600","1":"00:23:06.000","2":"696dda4be4b0e8ba829fafd9","3":"1341.6000000000001","isRemoved":false},{"0":"00:23:06.000","1":"00:23:38.000","2":"696dda4ce4b0e8ba829fafdb","3":"1386.0","isRemoved":false},{"0":"00:23:38.000","1":"00:24:24.240","2":"696dda4ce4b0e8ba829fafdd","3":"1418.0000000000002","isRemoved":false},{"0":"00:24:24.240","1":"00:24:53.960","2":"696dda4ce4b0e8ba829fafdf","3":"1464.24","isRemoved":false},{"0":"00:24:53.960","1":"00:25:19.400","2":"696dda4ce4b0e8ba829fafe1","3":"1493.96","isRemoved":false},{"0":"00:25:19.400","1":"00:25:57.840","2":"696dda4ce4b0e8ba829fafe3","3":"1519.4","isRemoved":false},{"0":"00:25:57.840","1":"00:26:29.000","2":"696dda4ce4b0e8ba829fafe5","3":"1557.8400000000001","isRemoved":false},{"0":"00:26:29.000","1":"00:28:04.040","2":"696dda4ce4b0e8ba829fafe7","3":"1589.0000000000002","isRemoved":false},{"0":"00:28:04.040","1":"00:29:56.520","2":"696dda4ce4b0e8ba829fafe9","3":"1684.0400000000002","isRemoved":false},{"0":"00:29:56.520","1":"00:31:30.560","2":"696dda4ce4b0e8ba829fafeb","3":"1796.5200000000002","isRemoved":false},{"0":"00:31:30.560","1":"00:33:15.920","2":"696dda4ce4b0e8ba829fafed","3":"1890.5600000000002","isRemoved":false},{"0":"00:33:15.920","1":"00:34:18.080","2":"696dda4ce4b0e8ba829fafef","3":"1995.92","isRemoved":false},{"0":"00:34:18.080","1":"00:34:46.640","2":"696dda4ce4b0e8ba829faff1","3":"2058.0800000000004","isRemoved":false},{"0":"00:34:46.640","1":"00:35:35.720","2":"696dda4ce4b0e8ba829faff3","3":"2086.6400000000003","isRemoved":false},{"0":"00:35:35.720","1":"00:37:36.560","2":"696dda4ce4b0e8ba829faff5","3":"2135.7200000000003","isRemoved":false},{"0":"00:37:36.560","1":"00:38:14.160","2":"696dda4ce4b0e8ba829faff7","3":"2256.5600000000004","isRemoved":false},{"0":"00:38:14.160","1":"00:38:39.680","2":"696dda4ce4b0e8ba829faff9","3":"2294.1600000000003","isRemoved":false},{"0":"00:38:39.680","1":"00:39:16.080","2":"696dda4ce4b0e8ba829faffb","3":"2319.6800000000003","isRemoved":false},{"0":"00:39:16.080","1":"00:40:39.480","2":"696dda4ce4b0e8ba829faffd","3":"2356.0800000000004","isRemoved":false},{"0":"00:40:39.480","1":"00:41:13.800","2":"696dda4ce4b0e8ba829fafff","3":"2439.48","isRemoved":false},{"0":"00:41:13.800","1":"00:41:33.840","2":"696dda4ce4b0e8ba829fb001","3":"2473.8","isRemoved":false},{"0":"00:41:33.840","1":"00:42:26.400","2":"696dda4ce4b0e8ba829fb003","3":"2493.84","isRemoved":false},{"0":"00:42:26.400","1":"00:43:08.160","2":"696dda4ce4b0e8ba829fb005","3":"2546.4","isRemoved":false},{"0":"00:43:08.160","1":"00:43:39.000","2":"696dda4ce4b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Learn how to quantify RNA-Seq reads mapped to a reference genome using RNASTAR in a step-by-step workflow.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:00:23.920","2":"696dc911e4b0e8ba829fae41","3":"0.0","isRemoved":false},{"0":"00:00:23.920","1":"00:02:03.160","2":"696dc911e4b0e8ba829fae43","3":"23.92","isRemoved":false},{"0":"00:02:03.160","1":"00:02:46.400","2":"696dc911e4b0e8ba829fae49","3":"123.16000000000001","isRemoved":false},{"0":"00:02:46.400","1":"00:03:41.680","2":"696dc911e4b0e8ba829fae4b","3":"166.4","isRemoved":false},{"0":"00:03:41.680","1":"00:04:11.160","2":"696dc911e4b0e8ba829fae4d","3":"221.68","isRemoved":false},{"0":"00:04:11.160","1":"00:04:44.920","2":"696dc911e4b0e8ba829fae4f","3":"251.16000000000003","isRemoved":false},{"0":"00:04:44.920","1":"00:05:06.840","2":"696dc911e4b0e8ba829fae51","3":"284.92","isRemoved":false},{"0":"00:05:06.840","1":"00:05:26.800","2":"696dc911e4b0e8ba829fae53","3":"306.84000000000003","isRemoved":false}][endslides]

This video covers RNA-Seq quantification using FeatureCounts, showing how to count reads mapped to a reference genome.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:01:17.120","2":"696dc486e4b0e8ba829fadc0","3":"0.0","isRemoved":false},{"0":"00:01:17.120","1":"00:01:55.280","2":"696dc486e4b0e8ba829fadc2","3":"77.12","isRemoved":false},{"0":"00:01:55.280","1":"00:02:35.400","2":"696dc486e4b0e8ba829fadc4","3":"115.28000000000002","isRemoved":false},{"0":"00:02:35.400","1":"00:03:08.240","2":"696dc486e4b0e8ba829fadc6","3":"155.4","isRemoved":false},{"0":"00:03:08.240","1":"00:04:14.720","2":"696dc486e4b0e8ba829fadc8","3":"188.24","isRemoved":false},{"0":"00:04:14.720","1":"00:04:54.200","2":"696dc486e4b0e8ba829fadca","3":"254.72000000000003","isRemoved":false},{"0":"00:04:54.200","1":"00:05:14.160","2":"696dc486e4b0e8ba829fadcc","3":"294.20000000000005","isRemoved":false}][endslides]

This tutorial demonstrates RNA-Seq mapping workflows, from interpreting MultiQC/Falco reports to choosing a mapper and evaluating the resulting alignments.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning 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Here we introduce the transcriptomics workflow, guiding you through RNA concepts, quantification, mapping, differential expression, and data visualization.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:01:01.033","2":"69270831e4b021481f770394","3":"0.0","isRemoved":false},{"0":"00:01:01.033","1":"00:02:39.900","2":"69270831e4b021481f770396","3":"61.03333333333333","isRemoved":false},{"0":"00:02:39.900","1":"00:03:42.633","2":"69270831e4b021481f770398","3":"159.9","isRemoved":false},{"0":"00:03:42.633","1":"00:04:19.900","2":"69270831e4b021481f77039a","3":"222.63333333333333","isRemoved":false},{"0":"00:04:19.900","1":"00:05:57.033","2":"69270831e4b021481f77039c","3":"259.9","isRemoved":false},{"0":"00:05:57.033","1":"00:07:15.033","2":"69270831e4b021481f77039e","3":"357.0333333333333","isRemoved":false},{"0":"00:07:15.033","1":"00:07:54.433","2":"69270831e4b021481f7703a0","3":"435.0333333333333","isRemoved":false},{"0":"00:07:54.433","1":"00:10:55.100","2":"69270831e4b021481f7703a2","3":"474.43333333333334","isRemoved":false},{"0":"00:10:55.100","1":"00:12:32.033","2":"69270831e4b021481f7703a4","3":"655.1","isRemoved":false},{"0":"00:12:32.033","1":"00:13:19.633","2":"69270831e4b021481f7703a6","3":"752.0333333333333","isRemoved":false},{"0":"00:13:19.633","1":"00:15:14.300","2":"69270831e4b021481f7703a8","3":"799.6333333333333","isRemoved":false},{"0":"00:15:14.300","1":"00:17:23.433","2":"69270831e4b021481f7703aa","3":"914.3","isRemoved":false},{"0":"00:17:23.433","1":"00:18:16.033","2":"69270831e4b021481f7703ac","3":"1043.4333333333334","isRemoved":false},{"0":"00:18:16.033","1":"00:21:49.900","2":"69270831e4b021481f7703ae","3":"1096.0333333333333","isRemoved":false},{"0":"00:21:49.900","1":"00:22:45.433","2":"69270831e4b021481f7703b0","3":"1309.9","isRemoved":false},{"0":"00:22:45.433","1":"00:23:45.300","2":"69270831e4b021481f7703b2","3":"1365.4333333333334","isRemoved":false},{"0":"00:23:45.300","1":"00:24:18.100","2":"69270831e4b021481f7703b4","3":"1425.3","isRemoved":false},{"0":"00:24:18.100","1":"00:25:54.100","2":"69270831e4b021481f7703b6","3":"1458.1","isRemoved":false},{"0":"00:25:54.100","1":"00:27:34.733","2":"69270831e4b021481f7703b8","3":"1554.1","isRemoved":false}][endslides]

A step-by-step tutorial on aligning reads to a reference genome, examining BAM file contents, and exploring results in a genome browser.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:01:54.160","2":"696cf3ece4b0e8ba829f9dec","3":"0.0","isRemoved":false},{"0":"00:01:54.160","1":"00:02:32.120","2":"696cf3ece4b0e8ba829f9dee","3":"114.16000000000001","isRemoved":false},{"0":"00:02:32.120","1":"00:03:22.240","2":"696cf3ece4b0e8ba829f9df0","3":"152.12","isRemoved":false},{"0":"00:03:22.240","1":"00:03:43.920","2":"696cf3ece4b0e8ba829f9df2","3":"202.24","isRemoved":false},{"0":"00:03:43.920","1":"00:07:35.960","2":"696cf3ece4b0e8ba829f9df4","3":"223.92000000000002","isRemoved":false},{"0":"00:07:35.960","1":"00:08:41.160","2":"696cf3ece4b0e8ba829f9dfc","3":"455.96000000000004","isRemoved":false},{"0":"00:08:41.160","1":"00:09:04.960","2":"696cf3ece4b0e8ba829f9dfe","3":"521.1600000000001","isRemoved":false},{"0":"00:09:04.960","1":"00:09:38.240","2":"696cf3ece4b0e8ba829f9e00","3":"544.96","isRemoved":false},{"0":"00:09:38.240","1":"00:10:04.840","2":"696cf3ece4b0e8ba829f9e02","3":"578.24","isRemoved":false},{"0":"00:10:04.840","1":"00:10:35.640","2":"696cf3ece4b0e8ba829f9e04","3":"604.84","isRemoved":false},{"0":"00:10:35.640","1":"00:11:08.680","2":"696cf3ece4b0e8ba829f9e06","3":"635.6400000000001","isRemoved":false},{"0":"00:11:08.680","1":"00:14:56.320","2":"696cf3ece4b0e8ba829f9e08","3":"668.6800000000001","isRemoved":false},{"0":"00:14:56.320","1":"00:15:31.840","2":"696cf3ede4b0e8ba829f9e0a","3":"896.32","isRemoved":false},{"0":"00:15:31.840","1":"00:18:08.040","2":"696cf3ede4b0e8ba829f9e0c","3":"931.84","isRemoved":false},{"0":"00:18:08.040","1":"00:18:39.280","2":"696cf3ede4b0e8ba829f9e0e","3":"1088.0400000000002","isRemoved":false},{"0":"00:18:39.280","1":"00:19:09.280","2":"696cf3ede4b0e8ba829f9e10","3":"1119.2800000000002","isRemoved":false},{"0":"00:19:09.280","1":"00:19:47.280","2":"696cf3ede4b0e8ba829f9e12","3":"1149.2800000000002","isRemoved":false},{"0":"00:19:47.280","1":"00:20:18.200","2":"696cf3ede4b0e8ba829f9e14","3":"1187.2800000000002","isRemoved":false},{"0":"00:20:18.200","1":"00:20:40.680","2":"696cf3ede4b0e8ba829f9e16","3":"1218.2","isRemoved":false},{"0":"00:20:40.680","1":"00:22:05.120","2":"696cf3ede4b0e8ba829f9e18","3":"1240.68","isRemoved":false},{"0":"00:22:05.120","1":"00:22:45.280","2":"696cf3ede4b0e8ba829f9e1a","3":"1325.1200000000003","isRemoved":false},{"0":"00:22:45.280","1":"00:24:02.560","2":"696cf3ede4b0e8ba829f9e1c","3":"1365.2800000000002","isRemoved":false},{"0":"00:24:02.560","1":"00:24:37.480","2":"696cf3ede4b0e8ba829f9e1e","3":"1442.5600000000002","isRemoved":false},{"0":"00:24:37.480","1":"00:27:02.720","2":"696cf3ede4b0e8ba829f9e20","3":"1477.48","isRemoved":false},{"0":"00:27:02.720","1":"00:27:27.000","2":"696cf3ede4b0e8ba829f9e22","3":"1622.72","isRemoved":false},{"0":"00:27:27.000","1":"00:27:56.640","2":"696cf3ede4b0e8ba829f9e24","3":"1647.0000000000002","isRemoved":false},{"0":"00:27:56.640","1":"00:28:37.720","2":"696cf3ede4b0e8ba829f9e26","3":"1676.64","isRemoved":false},{"0":"00:28:37.720","1":"00:29:07.120","2":"696cf3ede4b0e8ba829f9e28","3":"1717.72","isRemoved":false},{"0":"00:29:07.120","1":"00:29:42.720","2":"696cf3ede4b0e8ba829f9e2a","3":"1747.1200000000003","isRemoved":false},{"0":"00:29:42.720","1":"00:30:11.400","2":"696cf3ede4b0e8ba829f9e2c","3":"1782.7200000000003","isRemoved":false},{"0":"00:30:11.400","1":"00:31:28.920","2":"696cf3ede4b0e8ba829f9e2e","3":"1811.4","isRemoved":false},{"0":"00:31:28.920","1":"00:32:25.240","2":"696cf3ede4b0e8ba829f9e30","3":"1888.92","isRemoved":false},{"0":"00:32:25.240","1":"00:33:09.160","2":"696cf3ede4b0e8ba829f9e32","3":"1945.2400000000002","isRemoved":false},{"0":"00:33:09.160","1":"00:33:38.720","2":"696cf3ede4b0e8ba829f9e50","3":"1989.16","isRemoved":false},{"0":"00:33:38.720","1":"00:34:04.200","2":"696cf3ede4b0e8ba829f9e52","3":"2018.7200000000003","isRemoved":false},{"0":"00:34:04.200","1":"00:34:57.960","2":"696cf3ede4b0e8ba829f9e54","3":"2044.2000000000003","isRemoved":false},{"0":"00:34:57.960","1":"00:35:29.920","2":"696cf3ede4b0e8ba829f9e56","3":"2097.96","isRemoved":false},{"0":"00:35:29.920","1":"00:36:36.480","2":"696cf3ede4b0e8ba829f9e58","3":"2129.92","isRemoved":false},{"0":"00:36:36.480","1":"00:36:57.240","2":"696cf3ede4b0e8ba829f9e5a","3":"2196.48","isRemoved":false}][endslides]

Learn the key considerations for read mapping, from choosing the right tool for your data to understanding its impact and visualizing alignments in genome browsers.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:01:50.400","2":"696b5896e4b0e8ba829f82f1","3":"0.0","isRemoved":false},{"0":"00:01:50.400","1":"00:03:17.240","2":"696b5896e4b0e8ba829f82f3","3":"110.4","isRemoved":false},{"0":"00:03:17.240","1":"00:04:33.720","2":"696b5896e4b0e8ba829f82f5","3":"197.24","isRemoved":false},{"0":"00:04:33.720","1":"00:05:31.120","2":"696b5896e4b0e8ba829f82f7","3":"273.72","isRemoved":false},{"0":"00:05:31.120","1":"00:06:06.440","2":"696b5896e4b0e8ba829f82f9","3":"331.12","isRemoved":false},{"0":"00:06:06.440","1":"00:06:57.000","2":"696b5896e4b0e8ba829f82fb","3":"366.44000000000005","isRemoved":false},{"0":"00:06:57.000","1":"00:07:41.160","2":"696b5896e4b0e8ba829f82fd","3":"417.00000000000006","isRemoved":false},{"0":"00:07:41.160","1":"00:09:14.440","2":"696b5896e4b0e8ba829f82ff","3":"461.16","isRemoved":false},{"0":"00:09:14.440","1":"00:09:39.920","2":"696b5896e4b0e8ba829f8301","3":"554.44","isRemoved":false},{"0":"00:09:39.920","1":"00:10:42.040","2":"696b5897e4b0e8ba829f8303","3":"579.9200000000001","isRemoved":false}][endslides]

The video provides a step-by-step tutorial on evaluating FASTQ quality for short and long reads, performing quality correction, and consolidating quality metrics across datasets.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:01:22.400","2":"696b5809e4b0e8ba829f81fb","3":"0.0","isRemoved":false},{"0":"00:01:22.400","1":"00:02:06.633","2":"696b5809e4b0e8ba829f81fd","3":"82.4","isRemoved":false},{"0":"00:02:06.633","1":"00:02:36.466","2":"696b5809e4b0e8ba829f81ff","3":"126.63333333333334","isRemoved":false},{"0":"00:02:36.466","1":"00:03:13.966","2":"696b5809e4b0e8ba829f8201","3":"156.46666666666667","isRemoved":false},{"0":"00:03:13.966","1":"00:03:47.600","2":"696b5809e4b0e8ba829f8203","3":"193.96666666666667","isRemoved":false},{"0":"00:03:47.600","1":"00:04:53.566","2":"696b5809e4b0e8ba829f8205","3":"227.60000000000002","isRemoved":false},{"0":"00:04:53.566","1":"00:05:39.466","2":"696b5809e4b0e8ba829f8207","3":"293.56666666666666","isRemoved":false},{"0":"00:05:39.466","1":"00:06:30.966","2":"696b5809e4b0e8ba829f8209","3":"339.4666666666667","isRemoved":false},{"0":"00:06:30.966","1":"00:07:14.666","2":"696b5809e4b0e8ba829f820b","3":"390.9666666666667","isRemoved":false},{"0":"00:07:14.666","1":"00:07:48.733","2":"696b5809e4b0e8ba829f820d","3":"434.6666666666667","isRemoved":false},{"0":"00:07:48.733","1":"00:08:23.933","2":"696b580ae4b0e8ba829f820f","3":"468.73333333333335","isRemoved":false},{"0":"00:08:23.933","1":"00:08:49.700","2":"696b580ae4b0e8ba829f8211","3":"503.93333333333334","isRemoved":false},{"0":"00:08:49.700","1":"00:09:15.266","2":"696b580ae4b0e8ba829f8213","3":"529.7","isRemoved":false},{"0":"00:09:15.266","1":"00:09:58.533","2":"696b580ae4b0e8ba829f8215","3":"555.2666666666667","isRemoved":false},{"0":"00:09:58.533","1":"00:10:23.200","2":"696b580ae4b0e8ba829f8217","3":"598.5333333333334","isRemoved":false},{"0":"00:10:23.200","1":"00:11:37.200","2":"696b580ae4b0e8ba829f8219","3":"623.2","isRemoved":false},{"0":"00:11:37.200","1":"00:12:00.466","2":"696b580ae4b0e8ba829f821b","3":"697.2","isRemoved":false},{"0":"00:12:00.466","1":"00:12:23.700","2":"696b580ae4b0e8ba829f821d","3":"720.4666666666667","isRemoved":false},{"0":"00:12:23.700","1":"00:13:04.633","2":"696b580ae4b0e8ba829f821f","3":"743.7","isRemoved":false},{"0":"00:13:04.633","1":"00:14:43.266","2":"696b580ae4b0e8ba829f8221","3":"784.6333333333333","isRemoved":false},{"0":"00:14:43.266","1":"00:16:24.966","2":"696b580ae4b0e8ba829f8223","3":"883.2666666666668","isRemoved":false},{"0":"00:16:24.966","1":"00:17:06.933","2":"696b580ae4b0e8ba829f8225","3":"984.9666666666667","isRemoved":false},{"0":"00:17:06.933","1":"00:18:10.300","2":"696b580ae4b0e8ba829f823d","3":"1026.9333333333334","isRemoved":false},{"0":"00:18:10.300","1":"00:18:52.066","2":"696b580ae4b0e8ba829f823f","3":"1090.3","isRemoved":false},{"0":"00:18:52.066","1":"00:19:23.800","2":"696b580ae4b0e8ba829f8241","3":"1132.0666666666668","isRemoved":false},{"0":"00:19:23.800","1":"00:19:43.833","2":"696b580ae4b0e8ba829f8243","3":"1163.8","isRemoved":false},{"0":"00:19:43.833","1":"00:21:03.466","2":"696b580ae4b0e8ba829f8245","3":"1183.8333333333335","isRemoved":false},{"0":"00:21:03.466","1":"00:21:29.233","2":"696b580ae4b0e8ba829f8247","3":"1263.4666666666667","isRemoved":false},{"0":"00:21:29.233","1":"00:21:52.666","2":"696b580ae4b0e8ba829f8249","3":"1289.2333333333333","isRemoved":false},{"0":"00:21:52.666","1":"00:22:37.733","2":"696b580ae4b0e8ba829f824b","3":"1312.6666666666667","isRemoved":false},{"0":"00:22:37.733","1":"00:23:32.200","2":"696b580ae4b0e8ba829f824d","3":"1357.7333333333333","isRemoved":false},{"0":"00:23:32.200","1":"00:24:13.466","2":"696b580ae4b0e8ba829f824f","3":"1412.2","isRemoved":false},{"0":"00:24:13.466","1":"00:24:48.100","2":"696b580ae4b0e8ba829f8251","3":"1453.4666666666667","isRemoved":false},{"0":"00:24:48.100","1":"00:25:25.200","2":"696b580ae4b0e8ba829f8253","3":"1488.1000000000001","isRemoved":false},{"0":"00:25:25.200","1":"00:26:16.500","2":"696b580ae4b0e8ba829f8255","3":"1525.2","isRemoved":false},{"0":"00:26:16.500","1":"00:26:44.900","2":"696b580ae4b0e8ba829f8257","3":"1576.5","isRemoved":false},{"0":"00:26:44.900","1":"00:27:30.900","2":"696b580ae4b0e8ba829f8259","3":"1604.9","isRemoved":false},{"0":"00:27:30.900","1":"00:27:53.066","2":"696b580ae4b0e8ba829f825b","3":"1650.9","isRemoved":false},{"0":"00:27:53.066","1":"00:28:31.266","2":"696b580ae4b0e8ba829f825d","3":"1673.0666666666668","isRemoved":false},{"0":"00:28:31.266","1":"00:29:18.966","2":"696b580ae4b0e8ba829f825f","3":"1711.2666666666667","isRemoved":false},{"0":"00:29:18.966","1":"00:30:15.000","2":"696b580ae4b0e8ba829f8261","3":"1758.9666666666667","isRemoved":false},{"0":"00:30:15.000","1":"00:30:40.133","2":"696b580be4b0e8ba829f8263","3":"1815.0","isRemoved":false},{"0":"00:30:40.133","1":"00:31:40.300","2":"696b580be4b0e8ba829f8265","3":"1840.1333333333334","isRemoved":false},{"0":"00:31:40.300","1":"00:32:35.166","2":"696b580be4b0e8ba829f8267","3":"1900.3000000000002","isRemoved":false},{"0":"00:32:35.166","1":"00:32:56.966","2":"696b580be4b0e8ba829f8269","3":"1955.1666666666667","isRemoved":false},{"0":"00:32:56.966","1":"00:33:25.0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Good sequencing analysis begins and ends with quality control; set parameters wisely and re-run FastQC to verify results.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:01:06.680","2":"696a392ae4b0e8ba829f6f1a","3":"0.0","isRemoved":false},{"0":"00:01:06.680","1":"00:01:55.200","2":"696a392ae4b0e8ba829f6f1c","3":"66.68","isRemoved":false},{"0":"00:01:55.200","1":"00:16:18.720","2":"696a392ae4b0e8ba829f6f1e","3":"115.2","isRemoved":false},{"0":"00:16:18.720","1":"00:16:59.040","2":"696a392ae4b0e8ba829f6f20","3":"978.72","isRemoved":false},{"0":"00:16:59.040","1":"00:17:48.280","2":"696a392ae4b0e8ba829f6f22","3":"1019.0400000000001","isRemoved":false},{"0":"00:17:48.280","1":"00:18:10.160","2":"696a392ae4b0e8ba829f6f24","3":"1068.2800000000002","isRemoved":false},{"0":"00:18:10.160","1":"00:19:35.160","2":"696a392ae4b0e8ba829f6f26","3":"1090.16","isRemoved":false},{"0":"00:19:35.160","1":"00:23:19.960","2":"696a392ae4b0e8ba829f6f28","3":"1175.16","isRemoved":false}][endslides]

The goal of this exercise is to turn this list of genomic regions into a list of possible target genes.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning 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This is a short introduction to the Galaxy user interface - the web page that you interact with.

*For each of the sections covered in this course, please visit and follow along with the linked training materials. Additionally, you will find below a link to the history pad used during the live version of this course. Feel free to refer to it for exercises and Q&A, which might improve your learning experience.*[slides][{"0":"00:00:00.000","1":"00:00:43.000","2":"696a0633e4b0e8ba829f695a","3":"0.0","isRemoved":false},{"0":"00:00:43.000","1":"00:02:52.640","2":"696a0633e4b0e8ba829f695c","3":"43.0","isRemoved":false},{"0":"00:02:52.640","1":"00:03:15.960","2":"696a0633e4b0e8ba829f695e","3":"172.64000000000004","isRemoved":false},{"0":"00:03:15.960","1":"00:03:39.080","2":"696a0633e4b0e8ba829f6960","3":"195.96","isRemoved":false},{"0":"00:03:39.080","1":"00:04:11.040","2":"696a0633e4b0e8ba829f6962","3":"219.08","isRemoved":false},{"0":"00:04:11.040","1":"00:04:42.920","2":"696a0633e4b0e8ba829f6964","3":"251.04000000000002","isRemoved":false},{"0":"00:04:42.920","1":"00:06:02.760","2":"696a0634e4b0e8ba829f6966","3":"282.92","isRemoved":false},{"0":"00:06:02.760","1":"00:06:31.480","2":"696a0634e4b0e8ba829f6968","3":"362.76000000000005","isRemoved":false},{"0":"00:06:31.480","1":"00:07:42.560","2":"696a0634e4b0e8ba829f696a","3":"391.48","isRemoved":false},{"0":"00:07:42.560","1":"00:08:05.080","2":"696a0634e4b0e8ba829f696c","3":"462.56000000000006","isRemoved":false},{"0":"00:08:05.080","1":"00:08:44.200","2":"696a0634e4b0e8ba829f696e","3":"485.08000000000004","isRemoved":false},{"0":"00:08:44.200","1":"00:09:33.160","2":"696a0634e4b0e8ba829f6970","3":"524.2","isRemoved":false},{"0":"00:09:33.160","1":"00:09:54.040","2":"696a0634e4b0e8ba829f6972","3":"573.1600000000001","isRemoved":false},{"0":"00:09:54.040","1":"00:10:49.560","2":"696a0634e4b0e8ba829f6974","3":"594.0400000000001","isRemoved":false},{"0":"00:10:49.560","1":"00:11:16.800","2":"696a0634e4b0e8ba829f6976","3":"649.5600000000002","isRemoved":false},{"0":"00:11:16.800","1":"00:12:42.080","2":"696a0634e4b0e8ba829f6978","3":"676.8000000000001","isRemoved":false},{"0":"00:12:42.080","1":"00:13:13.160","2":"696a0634e4b0e8ba829f6984","3":"762.08","isRemoved":false},{"0":"00:13:13.160","1":"00:14:49.880","2":"696a0634e4b0e8ba829f698d","3":"793.1600000000001","isRemoved":false},{"0":"00:14:49.880","1":"00:16:02.280","2":"696a0634e4b0e8ba829f698f","3":"889.8800000000001","isRemoved":false},{"0":"00:16:02.280","1":"00:16:54.280","2":"696a0634e4b0e8ba829f6991","3":"962.2800000000001","isRemoved":false},{"0":"00:16:54.280","1":"00:18:45.960","2":"696a0634e4b0e8ba829f6993","3":"1014.2800000000001","isRemoved":false},{"0":"00:18:45.960","1":"00:19:23.960","2":"696a0634e4b0e8ba829f6995","3":"1125.96","isRemoved":false},{"0":"00:19:23.960","1":"00:20:03.360","2":"696a0634e4b0e8ba829f6997","3":"1163.96","isRemoved":false},{"0":"00:20:03.360","1":"00:20:50.640","2":"696a0634e4b0e8ba829f6999","3":"1203.3600000000001","isRemoved":false},{"0":"00:20:50.640","1":"00:21:39.920","2":"696a0634e4b0e8ba829f699b","3":"1250.64","isRemoved":false},{"0":"00:21:39.920","1":"00:22:10.840","2":"696a0634e4b0e8ba829f699d","3":"1299.92","isRemoved":false},{"0":"00:22:10.840","1":"00:22:45.600","2":"696a0634e4b0e8ba829f699f","3":"1330.8400000000001","isRemoved":false},{"0":"00:22:45.600","1":"00:23:17.240","2":"696a0634e4b0e8ba829f69a1","3":"1365.6000000000001","isRemoved":false},{"0":"00:23:17.240","1":"00:23:50.760","2":"696a0634e4b0e8ba829f69a3","3":"1397.24","isRemoved":false},{"0":"00:23:50.760","1":"00:24:23.760","2":"696a0634e4b0e8ba829f69a5","3":"1430.7600000000002","isRemoved":false},{"0":"00:24:23.760","1":"00:25:30.160","2":"696a0634e4b0e8ba829f69a7","3":"1463.7600000000002","isRemoved":false},{"0":"00:25:30.160","1":"00:26:12.040","2":"696a0634e4b0e8ba829f69a9","3":"1530.16","isRemoved":false}][endslides]