This course provides a comprehensive introduction to RNA-sequencing (RNA-seq) data analysis using the Galaxy platform. Galaxy offers an accessible, user-friendly, and FAIR environment that empowers researchers without programming experience to perform complex bioinformatics analyses. The course begins with an introduction to Galaxy, followed by tutorials on sequencing quality control and read mapping. Participants will then learn both the foundational and advanced steps of RNA-seq data analysis, including quantification and differential expression. The course concludes with an overview of relevant data types, databases, and resources to support further exploration and interpretation of RNA-seq results.
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